The objective of this study was to gain a better understanding of agronomic nitrogen (N) use efficiency and its effects on grain yield and quality of bread wheat landraces. This experiment was conducted in agricultural research center of Moghan, Iran, with 42 landraces at 3 replications and two N levels (0 and 200 kg ha-1), based on RCB-design. Analysis of variance for N levels was significant at P<0.01 for grain yield and at p<0.05 for Thousands Kernel Weight (TKW), grain N content, grain protein yield and seed hardness index. Genotypes showed high significant differences for all traits at (p≤0.01). Genotype×Nitrogen use efficiency (GN) interactions showed significant differences for all characters except grain yield and grain N content at p≤0.01. Reduction of N causes to diminishing in grain yield, grain N content, bread volume, but it caused increasing of TKW. The highest N Uptake has been shown in G19 and G29. Genotypes showed high genetic diversity in bread making quality characters. Evaluations of GN interactions, based on Wricke equivalent showed 40% of variance belongs to four G4, G5, G9 and G25 genotypes. Protein percent observed a positive and significant correlation with Zeleny Sedimentation, bread volume and grain N content. Cluster analysis classified genotypes in two main groups. Cluster one characterizes with high grain yield, TKW and bread volume and high Zeleny sedimentation, protein percent and grain N content were the second cluster`s characters.